Rarely. A properly-closed shipping container is essentially pest-proof — door gaskets seal, walls are solid steel, and there are no gaps a rodent can chew through. The only entry points are gaps you create (vents, conduit holes) or compromised gaskets.
Mice can squeeze through holes the diameter of a pencil; rats need a half-inch. A factory-spec shipping container has zero such holes. The doors gasket-seal when closed and latched. The floor is marine plywood on a steel frame — pests can't tunnel up. Pest entry is essentially a hardware failure.
Where pests do get in: aftermarket vents cut without proper screens, conduit / wire pass-throughs that weren't sealed, doors left ajar for extended periods, or gaskets that have dried/torn and lost their seal. Each is easy to inspect and fix.
Practical advice: when you receive a container, inspect the gaskets (look for cracks or hardened spots), check the floor for any cuts or compromised seams, and seal any vents with hardware cloth (1/4-inch mesh). Pet food, bird seed, and other rodent attractants should be in sealed metal containers inside — not directly on the floor.
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